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. 2013 Apr 29;8(4):e62297. doi: 10.1371/journal.pone.0062297

Figure 1. Transcript levels of lacZ transcribed from various rrn promoter regions.

Figure 1

The pACYC plasmids were constructed so that they contained various rrn operon promoter regions fused upstream a promoterless lacZ gene, as described in Materials and Methods. The short promoter region of rrn contained only the P1 sequence of the rrn promoter (S = short), the second segment contained the P1, P2 and nut -like sequences (boxA, boxB and boxC) (M = medium length) and a longer segment contained the P1, P2, nut like sequences and tL region of the rrn operon (L = long). All the promoter regions contained the FIS elements and other elements required for initiation of transcription. The pACYC plasmids were transformed into the lacZ and ybeY, lacZ deletion strains. (A) a schematic description of the three promoter regions which were fused to lacZ. The rrn sequence, the FIS elements, P1, P2, nut -like sequences and tL region are marked. (B) Cultures of ΔlacZ (“wild type ybeY”) and ΔlacZΔybeY (“ΔybeY”) carrying the pACYC plasmids were grown in LB at 37°C and harvested at O.D600 = 0.45. RNA was extracted from these samples and analyzed by qRTPCR.