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. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054

Figure 2. Additional flexibility in pMULTIrec.

Figure 2

A. Detailed schematic (not to scale) of att configuration and multiple cloning sites in pMULTIrec. B. Example of Gateway cassette exchange. Any cDNA of interest can be PCR-amplified cloned into the desired pDONR vector to generate a new pENTR clone. The same pDONR vector is used in a BP reaction with pMULTIrec, transformed in ccdB R bacteria and selected using Amp/Cam double selection. This generates a new DEST vector with the ENTR cassette in place of the desired fragment. This new cDNA is transferred to this new DEST vector via a standard LR reaction. C. Additional pMULTIrec variants we have generated.