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. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054

Figure 3. The Six2-GCiP BAC construct made using pMULTIrec.

Figure 3

A. The four-fragment and dual selection cassette from pMULTIrec was cloned in a Six2 containing BAC replacing the start codon. B. GFP imaging of a E13.5 embryo carrying the Six2-GCiP BAC. C. GFP imaging of whole mount E13.5 kidneys. D. E13.5 Six2-GCiP BAC kidneys cultured for four days showing GFP expression in the cap mesenchyme and Cdh1 expression in the ureteric bud.