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| Constructs | Colonies tested | Correct 5′ and 3′ PCRbased test for integration | Desired construct |
| Six2-eGFPCre-IRES-puro-pA+Six2-BAC | 10 | 10 | Six2-eGFPCre-IRES-puro-pA BAC |
| Nanog-mKate2-IRES-puro-pA+Nanog-BAC | 6 | 6 | Nanog-mKate2-IRES-puro-pA BAC |
