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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Cancer Biol Ther. 2005 Sep 23;4(9):1006–1013. doi: 10.4161/cbt.4.9.1970

Figure 5.

Figure 5

Effects of p53 siRNA on polyamine analogue-induced growth inhibition and apoptosis. (A). Vector and p53 siRNA transfected MCF-7 cells were treated with 1–10 μM polyamine analogue for 96 h and analyzed by MTT assays. Shown are means ± S.D. of three independent experiments performed in quadruplicate. There were statistically significant differences between transfected cells and vector transfected cells treated by >5 μM CGC-11144, BENSpm, CHENSpm and >1 μM CGC-11172. (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test). (B) Vector and p53 siRNA transfected MCF-7 cells were treated with 10 μM CGC-11144 for 96 h. Fragmented DNA was analyzed by electrophoresis in 1.2% agarose gel containing 0.1% ethidium bromide. Lane 1 is 1-kilobase DNA markers. The experiment was performed twice with similar results.