Fig. 3.

Correlation between the initial frequency of Vδ2⁺ T cells and the responsiveness of Vδ2⁺ T cells to Zol/IL-2 stimulation. a Correlation between the initial frequencies of Vδ2⁺ T cells and their numbers after Zol/IL-2 stimulation. b Correlation between the initial proportions of Vδ2⁺ T cells and their proportions after Zol/IL-2 stimulation. c, d Correlation between the initial frequency of Vδ2⁺ T cells and their production of IFN-γ (c) and TNF-α (d) after Zol/IL-2 stimulation. PBMC from 18 patients with breast cancer were stimulated with Zol/IL-2 for 10 days, the number of Vδ2⁺ T cells determined by means of dye exclusion and flow cytometry, and the data shown as a scatter plot. For production of IFN-γ and TNF-α, PMBC from 34 (c) or 36 (d) patients were stimulated with Zol/IL-2 for 2 days, the culture supernatants harvested, and IFN-γ or TNF-α levels determined by ELISA. The cytokine levels are plotted against the initial frequency of Vδ2⁺ T cells. Data obtained from early- and late-stage breast cancer patients were plotted as closed and open circles, respectively