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CDK9 foci colocalize with RNAPII-Ser5P but not with RNAPII-Ser2P. Deconvolved optical slice of a CDK9-mCherry MEF cell immunostained for RNAPII-Ser5P (A) or RNAPII-Ser2P (D). Volume-rendered images of A (B) and D (E) and zoom in C and F of insets of B and E. (G) Normalized frequency distribution of all of the colocalizing objects of RNAPII-Ser2P (gray line) and RNAPII-Ser5P (black line) with CDK9-mCherry. Bars show the percentage of noncolocalized staining. Bar, 4 μm.
