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CDK9 foci colocalize with Hexim1 and cyclin T1 but not with CDK12 and SC35. Deconvolved optical slice of CDK9-mCherry MEF cells derived from a CDK9-mCherry knock-in mouse. Cells were immunostained for CDK12 (A), Hexim1 (C), cyclin T1 (E), or SC35 (G). (B,D,F,H) Graphs show the percentage of noncolocalized staining (green bars) and the normalized frequency distribution of all colocalizing objects (gray line graphs) (see also the Material and Methods).
