Table 1.
Sequences of synthetic random-coil peptide standards.
| Series | Peptide Standard | Peptide Sequence | Net Charge pH 2 | Change in Carbon atom Content |
|---|---|---|---|---|
| S1–S5 | S1 | R–G–A–G–G–L–G–L–G–K–amide | +3 | 1 |
| S2 | Ac–R–G–G–G–G–L–G–L–G–K–amide | +2 | 0 | |
| S3 | Ac–R–G–A–G–G–L–G–L–G–K–amide | +2 | 1 | |
| S4 | Ac–R–G–V–G–G–L–G–L–G–K–amide | +2 | 2 | |
| S5 | Ac–R–G–V–V–G–L–G–L–G–K–amide | +2 | 3 | |
|
| ||||
| RX1–4 | RX1 | Ac–G–G–G–L–G–G–A–G–G–L–K–amide | +1 | – |
| RX2 | Ac–K–Y–G–L–G–G–A–G–G–L–K–amide | +2 | – | |
| RX3 | Ac–G–G–A–L–K–A–L–K–G–L–K–amide | +3 | – | |
| RX4 | Ac–K–Y–A–L–K–A–L–K–G–L–K–amide | +4 | – | |
|
| ||||
| HILIC 3 | 3a | G–G–G–G–K–L–G–L–G–K–amide | +3 | 0 |
| 3b | G–G–A–G–K–L–G–L–G–K–amide | +3 | 1 | |
| 3c | G–G–A–A–K–L–G–L–G–K–amide | +3 | 2 | |
| 3d | G–G–V–G–K–L–G–L–G–K–amide | +3 | 3 | |
| 3e | G–G–V–A–K–L–G–L–G–K–amide | +3 | 4 | |
| 3f | G–G–I–G–K–L–G–L–G–K–amide | +3 | 4 | |
| 3g | G–G–I–A–K–L–G–L–G–K–amide | +3 | 5 | |
| 3h | G–G–I–V–K–L–G–L–G–K–amide | +3 | 7 | |
| 3i | G–G–I–I–K–L–G–L–G–K–amide | +3 | 8 | |
Ac denotes Nα-acetyl; amide denotes Cα-amide. Variations in hydrophobicity of the peptide analogues for S1–S5 and HILIC 3 standards are indicated in bold. For the RX1 to RX4 standards the Lys residues are bolded.