Figure 5. CD1c⁺ DCs elicit the differentiation of CD103⁺CD8⁺ T cells in vivo.

(A–B) CD1c⁺ and CD141⁺ DCs were purified from human blood and cocultured with allogeneic naïve CFSE labeled CD8⁺ T cells. Proliferating CFSE⁻ CD8⁺ T cells cultured with either CD1c⁺ or CD141⁺ DCs were sorted and further labeled with CFSE. CFSE-relabeled T cells (1×10⁵ cells) were injected into the human epithelial grafts and single-cell suspensions were analyzed by flow cytometry at day 3. Dot plots show the presence of CD8⁺CFSE⁺ T cells. (B) Anti-CD103 or isotype control Abs (50 μg) were given daily to block T cell binding. (C) CD1c⁺ and CD141⁺ DCs subsets sorted from human blood were co-injected with allogeneic naïve CFSE-labeled T cells (3×10⁵ DCs and 3×10⁶ T cells) into human epithelial environments. Human epithelia were harvested 6 days later and analyzed by flow cytometry. Dot plots show the presence of CD3⁺CD8⁺ human T cells (upper plots) and the percentages of CFSE-negative CD103⁺ cells in gated CD3⁺CD8⁺ T cells (lower plots). One of two experiments shown. See also Figure S5.