Table 1.
Main experimental information used in the RSCN GRN.
| INTERACTIONS | EXPERIMENTAL EVIDENCE | REFERENCE |
|---|---|---|
| SHR → SCR | The expression of SCR is reduced in shr mutants background. ChIP-QRTPCR experiments show that SHR interacts in vivo with the predicted regulatory sequences of SCR and positively regulate it | Helariutta et al. (2000), Levesque et al. (2006), Cui et al. (2007) |
| SCR → SCR | In scr mutant background promoter activity of SCR is absent in the QC and CEI. A ChIP-PCR assay confirmed that SCR binds to its own promoter and promotes its own expression | Sabatini et al. (2003), Cui et al. (2007) |
| JKD → SCR | The SCR promoter expression in QC and CEI is not detected in JKD mutants from early heart stage onward. JKD was able to activate luciferase gene expression driven by a 1.5 kb SCR promoter region | Welch et al. (2007), Ogasawara et al. (2011) |
| JKD → JKD | JKD was able to activate luciferase gene expression driven by a 3.5 kb JKD promoter region | Ogasawara et al. (2011) |
| JKD → MGP | JKD was able to activate luciferase gene expression driven by a 3.5 kb MGP promoter region | Ogasawara et al. (2011) |
| MGP -| MGP | MGP addition was able to inhibit the SHR, SCR, and JKD induced luciferase gene expression driven by a 3.5 kb MGP promoter region | Ogasawara et al. (2011) |
| SHR → KD | The post-embryonic expression of JKD is reduced in shr roots. A CHIP-chip analysis detected JKD as a direct target gene of SHR | Welch et al. (2007), Cui et al. (2011) |
| SCR → JKD | The post-embryionic expression of JKD is reduced in scr roots | Welch et al. (2007) |
| SCR → WOX5 | WOX5 expression is reduced in shr mutants | Sarkar et al. (2007) |
| SHR → WOX5 | WOX5 expression is undetectable in scr mutants | Sarkar et al. (2007) |
| Auxin signalin pathway → WOX5 | In mp or bdl mutants background WOX5 expression is rarely detected | Sarkar et al. (2007) |
| Auxin signalin pathway -| WOX5 | In iaa17 mutants background WOX5 expression is decreased | Ding and Friml (2010) |
| SCR → miRNA165/6 | In scr single mutants, miRNA165/6 expression is greatly reduced. A ChIP-PCR assay confirmed that SCR binds to miRNA165/6 promoter | Carlsbecker et al. (2010), Miyashima et al. (2011) |
| SHR → miRNA165/6 | In shr single mutants, miRNA165/6 expression is greatly reduced. A ChIP-PCR assay confirmed that SHR binds to miRNA165/6 promoter | Carlsbecker et al. (2010), Miyashima et al. (2011) |
| miRNA165/6 -| PHB | Over expression of miRNA165/6 causes a decrease in the transcript levels of PHB. The allele phb-1d that expresses miRNA165/6-resistant PHB transcripts has ectopic PHB transcripts expression | Zhou et al. (2007), Miyashima et al. (2011) |
| PHB -| WOX5 | In se mutants, which fail to repress PHB expression, embryonic WOX5 expression is absent | Grigg et al. (2009) |
| PHB → JKD | jkd transcripts levels are reduced in the phb-1d miRNA-resistant PHB allele | Miyashima et al. (2011) |
| CLE40 → ACR | CLE40p treatment strongly increased ACR expression | Stahl et al. (2009) |
| CLE40 -| WOX5 | In cle40 mutants the WOX5 expression domain is expanded, and CLE40p treatment reduced WOX5 expression in the QC | Stahl et al. (2009) |
| SHR → MGP | The expression of MGP is severely reduced in the shr background. Experimental data using various approaches have suggested that MGP is a direct target of SHR. This result was later confirmed by ChIP-PCR | Levesque et al. (2006), Cui et al. (2007, 2011), Welch et al. (2007) |
| SCR → MGP | SCR directly binds to the MGP promoter, and MGP expression is reduced in the scr mutant background | Levesque et al. (2006), Welch et al. (2007) |