Figure 4.
Similar calcium responses are evoked by low and high Oxo-M concentrations. (A) Representative calcium responses of a cell transfected with M1Rs, loaded with Fura-4F-AM, and treated with 1 nM, 0.1 µM, or 10 µM Oxo-M. (B) Summary of Fura-4F amplitude, time to half-maximum, and duration for experiments as in A (n = 16). (C) Simulations from our kinetic model reproducing the observations in A. Quotation marks on Oxo-M are a reminder that this is a computer simulation rather than experiment. Assumed [Fura-4F] was 1 µM. (D) Simulation showing corresponding rises in cytosolic calcium concentration during 1 nM, 0.1 µM, and 10 µM Oxo-M. Fura-4F concentration was set at 0 µM.