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. 2013 May;141(5):633–647. doi: 10.1085/jgp.201210949

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© 2013 Shigetomi et al.

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Figure 4. — Ca²⁺ signals measured with Fluo-4, cyto-GCaMP3, and Lck-GCaMP3. (A) Representative images of an astrocyte loaded with Fluo-4AM and expressing Lck-GCaMP3 and cyto-GCaMP3. ROI are shown in each image, and their intensities are plotted below. (B) Cumulative probability plots for Ca²⁺ event peak response (i), T_0.5 (ii), and frequency (iii) for the indicated experimental groups. The distributions in each experimental condition were different between Lck-GCaMP3 and cyto-GCaMP3 on the one hand, and Fluo-4AM from control and mock (saline-injected) mice on the other (Kolmogorov–Smirnov test). (C and D) Summary graphs for Ca²⁺ signal properties for astrocyte somata and branchlets under the experimental conditions indicated. Data were gathered from n > 9 for each experimental condition. **, P < 0.01 when compared with an unpaired Student’s t test.