Fig 2.
Ex vivo target cell selection of the wild type and the ΔrfaL mutant. Spleens from three healthy 6- to 8-week-old C57BL/6 mice were harvested and homogenized. The homogenates were infected with the wild type carrying YopM-Bla or the ΔrfaL mutant carrying YopM-Bla at 37°C. Cells were stained with antibodies and CCF2-AM and then analyzed by flow cytometry. The experiment was done in triplicate, and data are pooled from the three replicates. Means and SEM are shown. (A) Overall distribution of spleen populations. The inset is a magnified view of the indicated populations. (B) Target cell injection. Individual cell types were evaluated to determine the percentage of injected cells within that population. Differences in injection between the WT and the rfaL mutant for individual cell types were determined by two-tailed Student's t tests. ***, P < 0.001; **, P = 0.001 to 0.01; *, P = 0.01 to 0.05. TH, T-helper cells; TC, T-cytotoxic cells; NK, natural killer cells; DC, dendritic cells; Mac, macrophages; Neut, neutrophils.