Table 2.
Primers used in this study
| Primer | Sequence (5′ → 3′) |
|---|---|
| RegR-F | GGGACCTGTTAAATGCATATGTAC |
| RegR-kanR | GAAGCAGCTCCAGCCTACACAGCATTTTGACTATTCATACAGTCACC |
| RegR-kanF | CTAAGGAGGATATTCATATGGCTTGAAAGTGAAAAGGAAATCATAATATG |
| RegR-R | CAGAACTCAATGGCGGCGATGTC |
| RegR-F1 | GGCCGAACCTCTGTATATGAG |
| RegR-R1 | CGTATCACCTGACACACTTCTC |
| RalR-F | GAGAAACGCAGAGCAGCCTAAA |
| RalR-kanR | GAAGCAGCTCCAGCCTACACAGCCCCTTCGCATTAGCCAAT |
| RalR-kanF | CTAAGGAGGATATTCATATGGGTCTGGGGTTGAATCAGTGCCT |
| RalR-R | CATTATCTGGCCATTTGCATCGTC |
| RalR-F1 | ACGCAGAGCAGCCTAAAACG |
| RalR-R1 | CAAGCCCCCCTCAAAAAT |
| RegR-BamHIF | CGGGATCCCGAGTTTACAGAGTGGATATCC |
| RegR-SalIR | CGGTCGACCAGTAAGAACTGCTTCTGCC |
| RegR-BamHIF | GGATCCATGAATAGTCAAAATGCTTTAAGTAATATGAGGCTTG |
| RegR-HindIIIR | AAGCTTTTATGATTTCCTTTTCACTTTCAAGGAAAACTGT |
| SefA-PE-F | CTCCCTAAATCCACTCATTCTTACTTA |
| SefA-PE-R | GCTGTTTCAGCGGCATTTGC |
| SefA_RegRB1_F | CCAATCAAAATAACAAAAAAACGCGCGTATACAAAACAA |
| SefA_RegRB1_R | TTGTTTTGTATACGCGCGTTTTTTTGTTATTTTGATTGG |
| SefA_RegRB2_F | CAAAACCAATAAAACACTCCACGCGCGTAAAAAAATATG |
| SefA_RegRB2_R | CATATTTTTTTACGCGCGTGGAGTGTTTTATTGGTTTTG |
| SefAP1-HindIII-F | GCCGAAGCTTTAGGCACTGACAATACGGCTGCAAACTT |
| SefAP-EcoRI-R | GCCGGAATTCTGGAGCCTGAACAGTAGCAACATCACCTAT |
| SefAFP2-HindIII-F | GCCGAAGCTTTAATGAAAATTCACTTAACCTAACGATTAAATTG |
| SefA-F | AAGTGTATGAGGTGCGTGGTCAGC |
| SefA-kanR | GAAGCAGCTCCAGCCTACACACCTGAACAGTAGCAACATCACCTATAGC |
| SefA-kanF | CTAAGGAGGATATTCATATGAGCAACATTCTACATTCAACAATATCAAGATTAA |
| SefA-R | TGGGGCTTTCGTCTTTTTATCG |
| SefA-F1 | CATCCATCCACAACTGGTTAG |
| SefA-R1 | CCAGACCGAAATCAGTTGAC |
| SefA-EMSA-F | TAGGCACTGACAATACGGCTGCAAACTT |
| SefA-EMSA-R | TTACAATGAAATAAAAACAATATAATTCACGCA |
| SefA-EMSAc-F | CTTATTCTTGCTTTGATGACGTGTG |
| SefA-EMSAc-R | ATCTCCCGTAACATAGACCCCTGC |
| pKD4F | TGTGTAGGCTGGAGCTGCTTC |
| pKD4R | CATATGAATATCCTCCTTAG |
| pKD4seqF | TGACGAGTTCTTCTGAGCGGGAC |
| pKD4seqR | TCTAGCTATCGCCATGTAAGCC |