Fig 5.

Evaluation of Nuc activity and protein levels across S. aureus strains. A collection of USA300 (11490, 18805 to 19321) and other S. aureus strains were grown in TSB for 15 h, and the cells were removed by filtration. As controls, WT LAC and the nuc mutant were included. Nuc enzyme activity and immunoblot assays of the spent medium were performed. Nuc activity was plotted with errors showing standard deviations, and immunoblot assay results for each sample are shown below the plot. See Table 1 for more information on the strains.