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Cells lacking Slr1 can form hyphae in liquid RPMI. Overnight YPD cultures of cells with the indicated genotypes were diluted to 105 cells/ml in RPMI and incubated on gelatin-coated coverslips for 1.5 (A) or 3 (B) h at 37°C and 5% CO2. Paraformaldehyde-fixed cells were imaged by differential interference contrast (DIC) microscopy. Scale bars = 5 μm.
