Fig 3.

Effects of DMPA treatment on serum cytokine concentrations in M. tuberculosis-infected C57BL/6 mice. Serum collected from C57BL/6 mice 3 and 8 weeks postinfection was subjected to cytokine measurement by Luminex analysis. DMPA-treated mice generally have lower levels of circulating cytokines than untreated mice. Pooled data from two independent experiments are shown. For each experiment, the serum of five mice in each of the infected groups (DMPA-M. tuberculosis and M. tuberculosis) and 3 of the naïve and DMPA-only groups were included for Luminex analysis. The log-transformed data were analyzed using a one-way ANOVA with a Fisher least significant difference (LSD) post hoc test and presented as least-squares means and 95% confidence intervals. A P value of <0.05 was regarded as significantly different (ns, not significant).