Fig 5.

DMPA treatment alters cytokine and chemokine secretion of PPD- and ESAT-6-stimulated MLN cells of C57BL/6 mice. C57BL/6 mice were injected intramuscularly with DMPA or saline weekly. Single cells were obtained from MLNs collected 3 (n = 5 mice/group) and 8 (n = 5 mice/group) weeks postinfection. Stimulations were done in triplicate in two independent experiments, and the cytokine levels were measured in the culture supernatant by Luminex analysis. DMPA treatment altered the ESAT-6 (A to D)- and PPD (E)-induced cytokine responses of the isolated MLN cells. Log-transformed data of two experiments were pooled and analyzed using a one-way ANOVA with a Fisher LSD post hoc test and presented as least-squares means and 95% confidence intervals. A P value of <0.05 was regarded as significantly different (ns, not significant).