Fig 8.

DMPA treatment does not alter the PPD-induced secretion of G-CSF, IP-10, and MIP-1α by MLN cells of BALB/c mice. BALB/c mice were injected intramuscularly with DMPA or saline weekly. Single cells were obtained from MLNs collected 3, 8, and 16 weeks postinfection from 5 mice/group. Stimulations were done in triplicate in two independent experiments, and cytokine levels were measured in the culture supernatants by Luminex analysis. DMPA treatment did not alter the PPD-induced G-CSF (A), IP-10 (B), and MIP-1α (C) responses of the isolated MLN cells. Log-transformed data from the two experiments were pooled and analyzed using a one-way ANOVA with a Fisher LSD post hoc test and presented as least-squares means and 95% confidence intervals. A P value of <0.05 was regarded as significantly different (ns, not significant).