Fig 6.

Histopathology of S. aureus SSTI lesions in RB6-8C5-treated or IgG2b-treated control mice. Ear pinnae were harvested from infected BALB/c mice that were treated with either RB6-8C5 to deplete neutrophils in vivo or an IgG2b isotype control. (A) Paraffin-embedded sections were stained with hematoxylin and eosin before examination under a light microscope. In contrast to control mice, neutrophil infiltration and abscess formation are not observed in neutrophil-depleted mice, but areas of necrosis are. (B) A lesion score of 1 to 5 was assigned by a blinded board-certified veterinary pathologist (n = 2 to 3 mice per group; performed in a single experiment; *, P < 0.05 compared to IgG2b controls by Student's t test). Bars represent SD. (C) Survival following S. aureus SSTI in RB6-8C5-treated versus isotype control mice (n = 6 to 8 mice per group; experiments performed in duplicate).