Table 1.

Primers used in this work

Primer name	Sequencea	Use	Restriction site(s)
324	TAT GGTCTCCCCGGG ACCATGCTGAACAATTATTAGCTC	Δspa	BsaI, XmaI
325	TAT GGTCTCACGCGT AATGTTTTTCTTTTTCAAATTAATACCC	Δspa	BsaI, MluI
326	TAT GGTCTCACGCGT CGTCGTCGCGAACTATAAAAACAAACAATACAC	Δspa	BsaI, MluI
354	TAT GGTCTCGGATCC TGTAGCAGCGAAACTTATTTCACC	Δspa	BsaI, BamHI
408	ATA GCCGGC AATAGTTACCCTTATTATCAAGATAAG	cat	NgoMIV
480	TAT GGTCTC AGATCCCGAAAACCGACTGTAAAAAGTACAG	cat	BsaI
476	TAT GGTCTC AGATCCTTATGAGCTAGAAAGGCTTATGCAG	Δsbi	BsaI
477	TAT GGTCTC TCGCGTCGAGATATATTTATTTTTCATGTGTATTCC	Δsbi	BsaI
478	TAT GGTCTC TCGCGTAATTTATTATCATTCTGGAAATAATCAATC	Δsbi	BsaI
479	TAT GGTCTC ATCGACTAAGCGTTTTCGAATTAACTGTTCG	Δsbi	BsaI
528	TAT GGTCTC AGATCCTTTCTATGTAATGGCAAAATTTATTCCCGACG	HlaH35L	BsaI
533	TAT GGTCTC ATCGACTTCCAATTTGTTGAAGTCC	HlaH35L	BsaI
alphaUSIE	TAT GGTCTC GCATGCCATTTTCTTTATCATAAGTG	HlaH35L	BsaI
alphaDSIE	TAT GGTCTC ACATGCTCAAAAAAGTATTTTATAG	HlaH35L	BsaI

^aRestriction sites are underlined.