Fig 1.

Deletion of mucR in Brucella abortus 2308 results in a growth deficiency. (A) Genetic organization of the mucR locus. The mucR gene is found on chromosome I and is designated bab1_0594 in the B. abortus 2308 genome sequence. mucR is flanked by ptrB (bab1_0593), encoding oligopeptidase B, and by a small, hypothetical-protein-encoding gene. (B) Growth curves of Brucella abortus strains in a rich medium. B. abortus 2308, the isogenic mucR mutant (ΔmucR), and the complemented mucR mutant (ΔmucR::pC³030) strains were grown in brucella broth, and the growth of each culture was monitored by plating serial dilutions on SBA to determine the number of CFU/ml. (C) Photograph of Brucella abortus colonies on blood agar after 60 h of growth.