Fig 3.

The lipopolysaccharide (LPS) core is modified in the B. abortus mucR mutant. Brucella strains were grown in brucella broth to stationary phase, and LPS was isolated by hot phenol extraction as described previously (33). Purified LPS from the specified Brucella strains was separated on an SDS-PAGE gel containing 3 M urea, and the LPS was then visualized by silver staining (A), Western blot analysis with an anti-O-chain monoclonal antibody (B), and Western blot analysis with an anticore monoclonal antibody (C).