Table 3.

Genes regulated by MucR in Brucella abortus 2308

Function and designation	Description	Fold change (ΔmucR strain vs 2308) in gene expression by:
		Microarraya	RT-PCR
Membrane proteins and transport systems
BAB1_0115	Outer membrane protein Omp25d	3.97
BAB1_0793	Membrane-bound proton-translocating pyrophosphatase	2.02
BAB1_1893	Hypb (DMEc family transporter)	14.69
BAB1_2138	Rare lipoprotein A	−2.07
BAB2_0055	Amino acid transporter	3.27
BAB2_0837	Hyp (polyferredoxin)	−2.34
BAB2_0838	Iron permease FTR1	−3.58
BAB2_0839	Hyp	−4.55
BAB2_0840	Membrane antigen (iron transport)	−3.03	−7.10
Transcription and translation
BAB1_0190	Transcriptional regulator, LuxR family protein BabR	21.06	73.82
BAB1_0460	Ribosomal protein P2	4.24
BAB1_0594	Transcriptional regulatory protein MucR	−99.95
BAB1_1605	Transcriptional regulator, LysR family protein ArsR6/NolR	2.10	2.36
BAB2_0143	AsnC family regulatory protein	2.02	2.62
BAB2_0806	LuxR family regulatory protein	2.69
BAB2_0807	Crp family regulatory protein	2.04	1.53
Polysaccharide biosynthesis and modification
BAB1_0326	Glycosyl transferase family protein	5.07
BAB1_0560	Phosphoglucomutase/phosphomannomutase	2.34
BAB1_1465	Glycoside hydrolase family protein	2.37
BAB1_1973	ExsB protein	−3.75	−3.31
BAB1_1974	Putative 6-pyruvoyl tetrahydropterin synthase	−2.98
BAB1_1975	Proline-rich extensin; radical SAMd family protein	−2.73
BAB2_0133	Glycosyltransferase family protein	2.96
BAB2_0134	Glu/Leu/Phe/Val dehydrogenase	2.99
BAB2_0135	Dolichyl-phosphate-mannose-protein mannosyltransferase	2.57
Genome plasticity
BAB1_0554	IS5 family transposase OrfA	2.81	104.68
BAB1_0555	IS5 family transposase OrfB	3.68
BAB1_0746	Hyp	91.29	1841.68
BAB1_0747	Integrase catalytic subunit	61.91
Metabolism, signaling, and enzymatic processes
BAB1_0459	Transglycosylase-associated protein	7.96
BAB1_0512	EALe domain-containing protein BpdB	4.30
BAB1_0655	Antifreeze protein	2.08
BAB1_0738	l-Lactate permease	−2.07
BAB1_1035	Proline-rich extensin	12.81	23.55
BAB1_1099	Patatin	3.20
BAB1_1206	7-Cyano-7-deazaguanine reductase	−2.38
BAB1_1511	Nudix hydrolase	2.13
BAB1_1535	C5 cytosine-specific DNA methylase	4.11
BAB1_2001	Aquaporin Z	3.28
BAB1_2010	Glyceraldehyde-3-phosphate dehydrogenase	14.23
BAB1_2041	ATP/GTP-binding domain-containing protein	21.01	120.32
BAB2_0196	Nickel-dependent hydrogenase b-type cytochrome subunit	2.82
BAB2_0257	Beta-lactamase	12.72
BAB2_0348	Protein kinase	−2.07
BAB2_0607	Twin-arginine translocation pathway signal	6.94
BAB2_0846	Frizzled protein	2.54
BAB2_0865	Pyridoxal-dependent decarboxylase	2.52
BAB2_0866	Glutamate decarboxylase alpha	2.21
BAB2_1072	PemK family protein	7.23
BAB2_1107	Aminoacyl-tRNA synthetase class I	9.88	7.21
Hypothetical
BAB1_0013	Hyp	3.29
BAB1_0043	Hyp	3.68
BAB1_0069	Hyp	6.57
BAB1_0070	Hyp	3.79
BAB1_0087	Hyp	2.19
BAB1_0189	Hyp	27.68
BAB1_0198	Hyp	2.31
BAB1_0265	Hyp	2.85
BAB1_0271	Hyp	4.47
BAB1_0324	Hyp	9.18
BAB1_0745	Hyp	7.09
BAB1_0750	Hyp	10.25
BAB1_0751	Hyp	2.31
BAB1_0893	Hyp	2.06
BAB1_1125	Hyp	5.91
BAB1_1352	Hyp	2.21
BAB1_1398	Hyp	−2.65
BAB1_1487	Hyp	2.62
BAB1_1489	Hyp	2.22
BAB1_1509	Hyp	3.47
BAB1_1529	Hyp	19.56
BAB1_1536	Hyp	2.48
BAB1_1604	Hyp	4.88
BAB1_1689	Hyp	2.34
BAB1_1854	Hyp	2.73	11.41
BAB1_2000	Hyp	3.15
BAB1_2002	Hyp	3.22
BAB1_2011	Hyp	10.97	573.77
BAB1_2012	Hyp	74.13
BAB1_2015	Hyp	−3.80
BAB1_2021	Hyp	7.01
BAB2_0092	Hyp	3.85
BAB2_0132	Hyp	2.79
BAB2_0258	Hyp	7.81
BAB2_0450	Hyp	2.84
BAB2_0613	Hyp	2.55
BAB2_0861	Hyp	6.12
BAB2_0867	Hyp	2.61
BAB2_0873	Hyp	2.04
BAB2_0887	Hyp	5.23
BAB2_1151	Hyp	−2.56

^aMicroarray analysis was performed using total cellular RNA from Brucella strains grown in brucella broth to late exponential phase, and values for genes whose expression was shown to be more than 2-fold altered in the mucR mutant compared to strain 2308 are shown.

^bHyp, hypothetical.

^cDME, drug/metabolite exporter.

^dSAM, S-adenosylmethionine.

^eEAL, cyclic di-GMP phosphodiesterase.