Fig 4.

CD11a regulates effector CD8 T cell differentiation. (A) KLRG1 and CD127 expression levels were used to subdivide Ova-K^b-specific CD8 T cells into EEC (KLRG1^low CD127^low), MPEC (KLRG1^low CD127^hi), DPEC (KLRG1^hi CD127^hi), and SLEC (KLRG1^hi CD127^low). (B and C) Representative zebra plots and bar graphs show CD62L versus PD-1 expression either among total Ova-K^b-specific CD8 T cells (B) or within specific effector subsets (C) in splenocytes of D8 infected mice. (D) Histogram displaying CD25 expression of antigen-specific CD8 T cells in tetramer-enriched splenocytes at day 4 from infected mice. Splenocytes from 3 mice were pooled per sample with a total of 12 mice per group. Bar graphs show frequenies and MFI values for CD25 expression in total tetramer-enriched CD8 T cells. The numbers on the zebra plots and histograms signify the percentages of cells that were within each gated region of representative samples. Data are representative of two or more experiments. Student's t test was used for statistical analysis. *, P < 0.05.