Fig 5.
Normal localization of Ova-Kb-specific CD8 T cells in CD11a−/− mice after L. monocytogenes infection. Thick sections of spleens were stained with Ova-Kb tetramer and antibodies to other surface markers to indicate B (B220) and T cell (CD8) zones and CD31 to identify blood vessels. (A) Images of uninfected spleens from WT mice were acquired with a 20× 0.75 numerical aperture (NA) objective. (B and C) Infection with 1 × 106 CFU ActA−/− L. monocytogenes Ova was used to assess the early T cell response (day 5 p.i.) in WT (B) and CD11a−/− (C) mice. (D and E) Sections from spleens of WT (D) and CD11a knockout (E) mice that had been infected with 1 × 103 CFU L. monocytogenes Ova 8 days prior. B, B cell zone; PALS, peri-arteriolar lymphoid sheath (T cell zone); RP, red pulp; CA, central arteriole.