Table 1.

Viable CFU in organs of CD4C/HIV^MutA Tg mice inoculated intranasally with Cryptococcus neoformans

Strain and variable	Valuea
	Tg mice			Control non-Tg mice
C. neoformans H99
Inoculum (CFU)	1.25 × 106	1.25 × 105	1.25 × 104	1.25 × 106	1.25 × 105	1.25 × 104
No. of mice inoculated	10	10	10	10	10	10
Mean (range) age at inoculation (days)	59 (49–63)	54 (50–57)	60 (50–64)	49 (42–63)	53 (45–67)	61 (50–64)
Mean (range) age at assessment (days)	72 (61–80)b	70 (64–75)b	80 (70–84)b	70 (58–80)b	76 (65–93)c	96 (73–106)c
Variables for organs culture positive for C. neoformans
Brain
No. of mice	7	9	9	10	8	5
C. neoformans count (CFU/g)	8.8 × 107	2.5 × 107	7.1 × 107	6.0 × 107	5.3 × 107	6.2 × 107
Range of counts	7.4 × 103-3.8 × 108	1.4 × 104-1.0 × 108	2.7 × 104-2.8 × 108	3.3 × 105-1.6 × 108	2.7 × 104-1.3 × 108	7.9 × 104-2.0 × 108
Lungs
No. of mice	9	10	9	10	9	6
C. neoformans count (CFU/g)	2.0 × 109	1.3 × 109	2.5 × 109	1.2 × 109	1.0 × 109	3.8 × 108
Range of counts	2.7 × 108-8.7 × 109	1.8 × 107-5.0 × 109	9.6 × 107-4.7 × 109	8.6 × 107-5.4 × 109	6.2 × 106-6.3 × 109	6.9 × 105-6.9 × 108
Liver
No. of mice	9	10	9	10	9	4
C. neoformans count (CFU/g)	4.6 × 105	1.3 × 106	3.5 × 105	2.9 × 105	8.8 × 104	7.3 × 104
Range of counts	2.5 × 104-2.5 × 106	4.2 × 104-7.6 × 106	1.3 × 105-6.5 × 105	2.9 × 104-8.4 × 105	1.1 × 104-3.2 × 105	1.9 × 104-2.1 × 105
Spleen
No. of mice	8	9	9	10	8	3
C. neoformans count (CFU/g)	3.6 × 106	1.5 × 106	1.5 × 106	4.0 × 105	2.2 × 105	6.0 × 104
Range of counts	1.1 × 105-2.2 × 107	2.3 × 105-2.8 × 106	3.6 × 105-4.0 × 106	5.4 × 104-1.1 × 106	1.8 × 104-6.5 × 105	9.1 × 103-1.2 × 105
C. neoformans C23
Inoculum (CFU)	1.25 × 106	1.25 × 105	1.25 × 104	1.25 × 106	1.25 × 105	1.25 × 104
No. of inoculated mice	10	10	10	10	10	10
Mean (range) age at inoculation (days)	53 (50–56)	53 (46–60)	46 (43–52)	45 (43–57)	49 (49–50)	52 (50–54)
Mean (range) age at assessment (days)	71 (62–77)b	105 (65–134)b	100 (53–135)d	90 (69–113)b	135 (81–142)d	140 (99–146)d
Variables for organs culture positive for C. neoformans
Brain
No. of mice	8	7	6	6	0	0
C. neoformans count (CFU/g)	2.2 × 107	4.4 × 106	8.1 × 106	1.2 × 107	NA	NA
Range of counts	3.6 × 104-7.1 × 107	5.9 × 103-2.0 × 107	9.5 × 103-1.4 × 107	8.2 × 105-4.3 × 107	NA	NA
Lungs
No. of mice	10	7	4	8	1	0
C. neoformans count (CFU/g)	4.2 × 108	1.3 × 108	2.2 × 108	5.7 × 108	4.4 × 106	NA
Range of counts	1.2 × 108-9.4 × 108	7.7 × 105-3.2 × 108	2.1 × 106-3.9 × 108	1.7 × 107-1.5 × 109	NA	NA
Liver
No. of mice	10	7	4	7	1	0
C. neoformans count (CFU/g)	6.1 × 105	6.0 × 105	2.2 × 106	3.4 × 106	4.4 × 103	NA
Range of counts	3.9 × 104-2.5 × 106	1.5 × 103-2.1 × 106	1.4 × 104-4.3 × 106	9.6 × 103-8.6 × 106	NA	NA
Spleen
No. of mice	10	5	4	5	0	0
C. neoformans count (CFU/g)	2.8 × 106	2.8 × 106	3.1 × 107	4.1 × 106	NA	NA
Range of counts	1.8 × 105-1.7 × 107	2.0 × 105-7.7 × 106	1.0 × 105-7.4 × 107	1.6 × 105-8.5 × 106	NA	NA

^aMice studied included Tg and control non-Tg offspring derived from the founder mouse F21388. NA, not applicable.

^bAssessment was done on the day of euthanization because of severe illness.

^cAssessment was done on the day of euthanization because of severe illness; survivors were euthanized 42 days after inoculation with C. neoformans.

^dAssessment was done on the day of euthanization because of severe illness; survivors were euthanized 92 days after inoculation with C. neoformans.