Table 3.
Plasmids used in this study
| Plasmid | Feature(s) | Source or reference |
|---|---|---|
| pKD46 | bla PBAD gam bet exo pSC101 ori(Ts), Ampr | 18 |
| pGEM-T Easy | Cloning vector, Ampr | Promega |
| pGEM-T::clpV | pGEM-T Easy harboring clpV, Ampr | This work |
| p3174 | Plasmid encoding the gfpmut3 gene | 22 |
| pSUB11 | 3×FLAG gene fusions plasmid, oriR6K, Kanr | 21 |
| pCP20 | bla cat cI857 λPR flp pSC101 ori(Ts), Camr, Ampr | 69 |
| pCE36 | aph FRT lacZY+ this oriR6K, Kanr | 20 |
| pCLF2 | Red-swap-redesigned vector, Camr | 70 |
| pCLF4 | Red-swap-redesigned vector, Kanr | 71 |
| pWRKC | Derivative of pir-dependent plasmid pNFB9, integrates at Gifsy-1 attachment site attG1, Camr | This work |
| pmCherry | Constitutive production of the mCherry fluorescent protein, Ampr | C. Marolda |
| pFlagTEM-1 | Plasmid used to construct translational fusions to the mature TEM-1 β-lactamase reporter | 26 |
| pSipA/FT | Plasmid which encodes the translational fusion between the mature TEM-1 β-lactamase reporter and SipA (STM2882) | 26 |
| pVgrG/FT | Plasmid which encodes the translational fusion between the mature TEM-1 β-lactamase reporter and VgrG (SG1044) | This work |