Figure 5.

Slug enhanced tumor growth but sensitized cells to vinorelbine in vivo. (A) Tumor volumes of A549/EGFP or A549/Slug tumors treated with the vehicle control or vinorelbine. A total of 4 × 10⁶ A549/EGFP or A549/Slug cells were subcutaneously inoculated into the right flank of each mouse (A549/EGFP, n = 10; A549/Slug, n = 10). Two weeks after inoculation, the mice were then treated with vinorelbine (n = 5, 5 mg/kg/week, i.p.) or the vehicle control (n = 5, PBS, i.p.) once a week for 3 weeks. The tumor volume was calculated as the length × width² × 0.5 and was assessed every 2–3 days. *P < 0.05. (B) HE, Slug, E-cadherin, βIII-tubulin, and βIV-tubulin staining of tumor specimens inoculated with A549/EGFP or A549/Slug cells. E-cad: E-cadherin. Original images were captured at ×200 magnification except for HE staining which was captured at ×40 magnification. EGFP, enhanced green fluorescent protein; PBS, phosphate-buffered saline.