Figure 2.

Immune infiltration of brain melanoma metastases in mice. (A–D) Immunofluorescence staining with anti-CD3 (green) and anti-F4/80 (red) Abs. Nuclei were stained with Dapi-Fluoromount-G (blue). Fluorescence analysis showed the same pattern of infiltration of CD3⁺ and F4/80⁺ predominantly at the tumor front (indicated by the dashed line). (E–H) Similar immunostaining in the tumor core, showing reduced immunocytes infiltration. (I) Curves representing quantitative image analysis of the immunofluorescence intensity of CD3⁺ and (J) F4/80⁺ cells. The numbers denote mean fluorescence intensity (MFI). Data are representative of three independent experiments (P < 0.05). (K) Histogram showing quantification of CD3⁺ cells (mean ± SEM; n = 8). (L) Histogram showing quantification of F4/80⁺ cells. (M) Histogram showing quantification of CD4⁺ and CD8⁺ cells within tumor front and tumor core (mean ± SEM; n = 6). (N) An illustrative depiction of the tumor regions. Red circle indicates the tumor core, and green circle the tumor front. All scale bars are 100 μm.