Figure 4.

Effect of crude extract, fractions and compound of M. alba on chemotaxis and cell viability. (A) Jurkat cells were pre-treated with vehicle (DMSO), the crude extract (CE, 10 μg/ml), butanol fraction (BuOH, 4 μg/ml), ethyl acetate fraction (EA, 4 μg/ml) and water fraction (H₂O, 10 μg/ml) of M. alba for 24 h. The cells were treated with PBS and SDF-1, respectively, for an additional 4 h in transwell microplates. The number of cells in the bottom well was counted. Cell migration is indicated as migration index (%), as defined in Materials and methods. (B &C) Jurkat cells were pre-treated with oxyresveratrol at 1, 2.5 and 5 μg/ml. The cells underwent chemotaxis assays (B) or WST-1 test (C) for cell viability. P (*) < 0.05 is considered statistically significant.