Table 3.
PCR primer and pooling factor information for the GS Junior HLA typing assay
| Locus | Exon | Reference |
Forward primer |
Reverse primer |
Amplicon length | Pool | Pooling Factor | ||
|---|---|---|---|---|---|---|---|---|---|
| Region | Position | Region | Position | ||||||
| A |
2 |
A*01:01:01:01 |
Exon 1 |
51-67 |
Intron 2 |
66-83 |
576 |
long |
1 |
| A |
3 |
A*01:01:01:01 |
Intron 2 |
150-171 |
Intron 3 |
65-83 |
521 |
long |
1 |
| A |
4 |
A*01:01:01:01 |
Intron 3 |
539-559 |
Intron 4 |
76-91 |
541 |
long |
1 |
| B |
1 |
B*07:02:01 |
5’NCR |
4998-5018 |
Intron 1 |
45-59 |
278 |
short |
0.5 |
| B |
2 |
B*07:02:01 |
Intron 1 |
45-59 |
Intron 2 |
25-43 |
467 |
long |
1 |
| B |
3 |
B*07:02:01 |
Intron 2 |
181-195 |
Intron 3 |
36-52 |
462 |
long |
1 |
| B |
4 |
B*07:02:01 |
Intron 3 |
501-519 |
Exon 5 |
27-45 |
558 |
long |
1 |
| C |
1 |
C*01:02:01 |
5’NCR |
210-230 |
Intron 1 |
42-62 |
279 |
short |
0.5 |
| C |
2 |
C*01:02:01 |
Intron 1 |
42-62 |
Intron 2 |
42-58 |
487 |
long |
1 |
| C |
3 |
C*01:02:01 |
Intron 2 |
145-165 |
Intron 3 |
65-83 |
531 |
long |
1 |
| C |
4 |
C*01:02:01 |
Intron 3 |
444-461 |
Intron 4 |
76-94 |
584 |
long |
1 |
| C |
7 |
C*01:02:01 |
Intron 6 |
62-80 |
Exon 8 |
14-33 |
366 |
short |
0.5 |
| DRB |
2 |
DRB1*01:01:01 |
Intron 1 |
5168-5189 |
Exon 2 |
250-270 |
545 |
long |
6 |
| DRB |
3 |
DRB1*01:01:01 |
Intron 2 |
2143-2163 |
Intron 3 |
1-20 |
459 |
long |
1 |
| DQB1 |
2 |
DQB1*02:01:01 |
Intron 1 | Exon 2 |
1429-1438 | 1-9 |
Exon 2 | Intron 2 |
268-270 | 1-16 |
356 |
short |
0.5 |
| DQB1 |
3 |
DQB1*02:01:01 |
Exon 3 |
43-62 |
Intron 3 |
1-27 |
357 |
short |
0.5 |
| DPB1 | 2 | DPB1*03:01:01 | Intron 1 | 4490-4517 | Intron 2 | 3-27 | 408 | long | 1 |