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. 2013 Apr 30;8(4):e62865. doi: 10.1371/journal.pone.0062865

Table 2. Comparison of the expression change of the 22 selected candidate genes with RNA-seq and qRT-PCR analysis.

Putative Function IDs Putative Function Fold change
RNA-seq qRT-PCR
Contig31917 taxane 2α-O-benzoyltransferase, TBT 17.1 6.9
Contig32749 taxane 7β-hydroxylase, T7OH 48.5 4.7
Contig30864 taxadien-5α-ol-O-acetyl transferase, TAT 34.3 19.2
Paclitaxel biosynthesis Contig28471 3′-N-debenzoyl-2′-deoxytaxol N-benzoyltransferase, DBTNBT 52.0 14.2
Contig19765 cytochrome P450 taxadiene 5α-hydroxylase, T5OH 29.9 3.3
Contig20518 10-deacetylbaccatin III-10-O-acetyl transferase, DBAT 21.1 3.9
247064 taxa-4(5),11(12)-diene synthase, TS 17.1 1.8
240648 baccatin III 3-animo-3-phenylpropanoyltransferas, BAPT 14.9 1.5
Contig30915 taxane 10β-hydroxylase, T10OH 5.7 3.6
HD2 type histone deacetylase 120154 HD2 type histone deacetylase, HD2 0.8 0.4
ACC oxidase Contig16235 ACC oxidase, ACC-1 4.6 4.4
235821 1-aminocyclopropane-1-carboxylate oxidase, ACC-2 24.3 5.4
Contig787# cell cycle switch protein CCS52a, CCS52a 4.9 0.5
Cell cycle Contig18564 cell cycle control protein cwf22, cwf22 0.4 0.6
114663 cell cycle checkpoint protein RAD17-like, RAD17 0.5 0.4
Candidates in the Contig24302 short chain alcohol dehydrogenase, ADH 14.9 14.8
unknown steps of 133463# AMP dependent CoA ligase, 4CL 26.0 1.7*
paclitaxel biosynthesis Contig24891 cytochrome P450 716B2, P450 12.1 17.0
Taxol degradation Contig17145 alpha/beta hydrolase fold-containing protein, abH 0.1 0.1
84500 carboxylesterase 17, CES17 0.1 0.2
Taxol transport Contig20317 ABC transporter I family member 6, chloroplastic, ABC-I 2.8 2.3
241293 ABC transporter G family member 3, ABC-G n.d. 0.2

#” indicate different expression models in RNA-seq analysis and qRT-PCR assays. “n.d.” indicates that the expression ratio of the gene in elicited cells to that in nonelicited cells approaches to zero. “*” indicates that the p-value is 0.447.