Table 2. Comparison of the expression change of the 22 selected candidate genes with RNA-seq and qRT-PCR analysis.
Putative Function | IDs | Putative Function | Fold change | |
RNA-seq | qRT-PCR | |||
Contig31917 | taxane 2α-O-benzoyltransferase, TBT | 17.1 | 6.9 | |
Contig32749 | taxane 7β-hydroxylase, T7OH | 48.5 | 4.7 | |
Contig30864 | taxadien-5α-ol-O-acetyl transferase, TAT | 34.3 | 19.2 | |
Paclitaxel biosynthesis | Contig28471 | 3′-N-debenzoyl-2′-deoxytaxol N-benzoyltransferase, DBTNBT | 52.0 | 14.2 |
Contig19765 | cytochrome P450 taxadiene 5α-hydroxylase, T5OH | 29.9 | 3.3 | |
Contig20518 | 10-deacetylbaccatin III-10-O-acetyl transferase, DBAT | 21.1 | 3.9 | |
247064 | taxa-4(5),11(12)-diene synthase, TS | 17.1 | 1.8 | |
240648 | baccatin III 3-animo-3-phenylpropanoyltransferas, BAPT | 14.9 | 1.5 | |
Contig30915 | taxane 10β-hydroxylase, T10OH | 5.7 | 3.6 | |
HD2 type histone deacetylase | 120154 | HD2 type histone deacetylase, HD2 | 0.8 | 0.4 |
ACC oxidase | Contig16235 | ACC oxidase, ACC-1 | 4.6 | 4.4 |
235821 | 1-aminocyclopropane-1-carboxylate oxidase, ACC-2 | 24.3 | 5.4 | |
Contig787# | cell cycle switch protein CCS52a, CCS52a | 4.9 | 0.5 | |
Cell cycle | Contig18564 | cell cycle control protein cwf22, cwf22 | 0.4 | 0.6 |
114663 | cell cycle checkpoint protein RAD17-like, RAD17 | 0.5 | 0.4 | |
Candidates in the | Contig24302 | short chain alcohol dehydrogenase, ADH | 14.9 | 14.8 |
unknown steps of | 133463# | AMP dependent CoA ligase, 4CL | 26.0 | 1.7* |
paclitaxel biosynthesis | Contig24891 | cytochrome P450 716B2, P450 | 12.1 | 17.0 |
Taxol degradation | Contig17145 | alpha/beta hydrolase fold-containing protein, abH | 0.1 | 0.1 |
84500 | carboxylesterase 17, CES17 | 0.1 | 0.2 | |
Taxol transport | Contig20317 | ABC transporter I family member 6, chloroplastic, ABC-I | 2.8 | 2.3 |
241293 | ABC transporter G family member 3, ABC-G | n.d. | 0.2 |
“#” indicate different expression models in RNA-seq analysis and qRT-PCR assays. “n.d.” indicates that the expression ratio of the gene in elicited cells to that in nonelicited cells approaches to zero. “*” indicates that the p-value is 0.447.