Figure 2. BAIC treatment reduced airway remodeling changes.

A) Periodic acid staining was performed with lung tissue sections and airway mucin (iv) was estimated by quantitative morphometry. B) Masson Trichrome staining was performed with lung tissue sections and sub-epithelial collagen content (iv) was estimated by quantitative morphometry. C) IHC was performed in lung tissue sections to determine the expression of TGF-β₁. Brown color indicates the positive expression of TGF-β₁. C, iv) The levels TGF-β₁ in lung tissue homogenates were determined by ELISA. All Images are at 20 X magnifications. Results were mean ± SEMs of three independent experiments. *P<0.05 vs. SHAM/PBS/VEH, ^†P<0.05 vs. OVA/OVA/VEH group. Data were mean ± SEM of three independent experiments. *P<0.05 vs. SHAM/PBS/VEH, ^†P<0.05 vs. OVA/OVA/VEH group. The diameters of bronchi which have been taken for morphometry analysis were 326.9±16.0 µm, 339.6±23.3 µm, and 326.4±47.3 µm for SHAM/PBS/VEH, OVA/OVA/VEH and OVA/OVA/BAIC, respectively.