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. 2013 Apr 30;8(4):e62907. doi: 10.1371/journal.pone.0062907

Figure 2. The relative levels of NbSTKL expression and BaMV accumulation in NbSTKL-knockdown N. benthamiana plants.

Figure 2

A. The expression levels of NbSTKL was determined by semi-quantitative RT-PCR (low panel) and actin was used for an internal control. Average from three experiments is shown in the top panel. Actin was used for normalization of each sample. The expression of NbSTKL in Luciferase-knockdown (siLuc) plants was set as 100%. B. The accumulation of BaMV coat protein was detected by Western blotting and normalized to the amount of RuBisCO large subunit (rbcL). The samples harvested from 5 dpi control (siLuc) plants were set as 100%. Results were from three independent experiments. The standard errors are shown. Asterisks indicate statistically significant differences between samples indicated (*p<0.05, **p<0.01).