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. 2013 Apr 30;8(4):e63177. doi: 10.1371/journal.pone.0063177

Figure 1. Primary cultures of chick gizzard cells express specific smooth muscle markers.

Figure 1

Cells were grown for 24 h, fixed and immunostained for the smooth muscle markers caveolin-3 (green, A and D), tropomyosin (red, B and E), and alpha-actin (green, C and F). Arrow in (D) point to a filamentous distribution of caveolin-3, arrows in (E) and (F) points to stress fibers. Note that the majority of the cells in each field are positive for smooth muscle alpha-actin (C), caveolin-3 (A), and tropomyosin (B). Scale bars = 20 µm.