Figure 2. Lack of Ang II production or AT₁ blockade impaired IFN-γ and IL-17 cytokine production by splenic CD4⁺ T cells during P.berghei ANKA infection.

T cells from naive mice and mice infected with P. berghei ANKA treated with vehicle, losartan or captopril by gavage were isolated at day 6 post infection, stained with fluorescent antibodies and analyzed by flow cytometry. Representative dot plots of IFN-γ⁺CD4⁺ (A) or IL-17⁺CD4⁺ (D) T cells obtained from gated CD3⁺ cells. The percentage (B) and absolute number of IFN-γ⁺CD4⁺ (C) and the percentage (E) and absolute number of IL-17⁺CD4⁺ (F) T cells were determined. The results are expressed as means±SD. Statistically significant compared with values for *naive mice (p<0.05) and #vehicle-treated mice infected with P. berghei ANKA (p<0.05).