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. 2013 Apr 30;8(4):e62999. doi: 10.1371/journal.pone.0062999

Figure 6. Effect of Ang II on adhesion of T lymphocytes to murine-activated endothelial cells (tEnd.1 cells) during P.berghei ANKA infection.

Figure 6

T lymphocytes from naive mice and mice infected with P. berghei ANKA treated with vehicle, losartan or captopril by gavage were isolated at day 6 post infection and co-cultured with TNF-α-activated tEnd.1 cells for 1 h. In addition, T lymphocytes from vehicle-treated mice infected with P. berghei ANKA were treated in culture with 10–6 M losartan or 10–6 M captopril for 30 min prior to co-incubation with TNF-α-activated tEnd.1 cells. (A) Representative microscope images showing endothelial cells containing T cells adhered after Diff-Quick staining. (B) The percentage of adherent cells described as the number of tEnd.1 cells containing lymphocytes in relation to the total number of tEnd.1 cells under each condition, after analysis of at least 10 random microscopic fields. The results are expressed as means±SE. Statistically significant compared with values for *naive mice (p<0.05) and #vehicle-treated mice infected with P. berghei ANKA (p<0.05).