Figure 6. Acetate and pH induces respectively a down-regulation of cyclin D1 and cyclin E1 proteins.

Western blots were performed after 16 hr co-culture of m-ICcl2 cells with 20 mM of acetate and lactate at different pHs. For the detection of cyclin E1, cells were synchronized by a double thymidine block treatment prior co-culture as described in material and methods section.