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. 2013 Apr 30;8(4):e62735. doi: 10.1371/journal.pone.0062735

Figure 4. pvcA mutation reduces the expression of cupB and cupC gene clusters.

Figure 4

The relative level of expression for each gene in PW4830 (ΔpvcA) compared to MPAO1 was determined by RT-qPCR. The quantity of cDNA in different samples was normalized using 30S ribosomal RNA (rplS) as an internal standard. A. pvcA mutation in MPAO1 significantly reduces the expression of all genes of the cupB gene cluster. Order and direction of transcription of cupB1-6 on the MPAO1 chromosome is indicated by block arrows above the graph. B. pvcA mutation in MPAO1 significantly reduces the expression of all cupC genes. Order and direction of transcription of cupC1-3 on the MPAO1 chromosome is indicated by block arrows above the graph. Values in A and B represent the average of triplicate PCR experiments conducted on three independently obtained RNA preparations ± SEM (n = 3); P<0.01 (**); P<0.001 (***).