. 2013 Apr 30;8(4):e62735. doi: 10.1371/journal.pone.0062735

Table 1. Bacterial strains and plasmids used in this study.

Strain or Plasmid	Description	Reference/Source
Strains
MPAO1	P. aeruginosa prototrophic laboratory strain	[66], [67], UWGC
PW4830	pvcA-F05::ISlacZ/hah; out of frame fusion in MPAO1; Tet^r	[67], UWGC
PW4832	pvcB-G05::ISlacZ/hah; out of frame fusion in MPAO1; Tet^r	[67], UWGC
PW4833	ptxR-P08::ISlacZ/hah; out of frame fusion in MPAO1; Tet^r	[67], UWGC
PW6105	rocS1-P09:ISlacZ/hah; out of frame fusion in MPAO1; Tet^r	[67], UWGC
PW7672	rocS2-P07::ISlacZ/hah; out of frame fusion in MPAO1; Tet^r	[67], UWGC
MPAO1ΔpvcCD	MPAO1 carrying a 600-bp BglII deletion encompassing portions of pvcC and pvcD; Tet^r	This study
Plasmids
pCR®2.1-TOPO®	General cloning vector; Cb^r, Km^r	Invitrogen
pCR2.1-1.8	pCR®2.1-TOPO® carrying the 1.8-kbp PstI P. aeruginosa stability fragment; Cb^r, Km^r	This study
pLL1	pCR2.1-1.8 carrying intact pvcA on a 1730-bp fragment from MPAO1; Cb^r, Km^r	This study
pLL2	pCR2.1-1.8 carrying intact pvcB on a 1200-bp fragment from MPAO1; Cb^r, Km^r	This study
pLL4	pCR2.1-1.8 carrying intact rocS1 on a 4060-bp fragment from MPAO1; Cb^r, Km^r	This study
pLL5	pCR2.1-1.8 carrying intact pvcAB on a 2930-bp fragment from MPAO1; Cb^r, Km^r	This study
p18.230	pKT230 carrying pUC18; Cb^r, Km^r	[39]
pJAC7-1	pUC19 containing ptxR on a 2.1-kbp KpnI-BglII fragment carried in pKT230; Cb^r, Km^r	[39]
pMRP9-1	pUCP18 carrying a gene encoding enhanced green fluorescent protein (GFP); Cb^r	[43]
pAH54	pUC18 carrying a 5.4 kb HindIII-EcoRI fragment carrying ptxR, pvcD, pvcC, and part of pvcB	[39]
pNC80	pAH54 with a Tet cassette replacing the 600 bp BglII fragment that carries the entire pvcDand part of pvcC; Tet^r	This study

Cb, carbenicillin;

Km, kanamycin;

, resistant;

Tet, tetracycline.