Table 1. Bacterial strains and plasmids used in this study.
Strain or Plasmid | Description | Reference/Source |
Strains | ||
MPAO1 | P. aeruginosa prototrophic laboratory strain | [66], [67], UWGC |
PW4830 | pvcA-F05::ISlacZ/hah; out of frame fusion in MPAO1; Tetr | [67], UWGC |
PW4832 | pvcB-G05::ISlacZ/hah; out of frame fusion in MPAO1; Tetr | [67], UWGC |
PW4833 | ptxR-P08::ISlacZ/hah; out of frame fusion in MPAO1; Tetr | [67], UWGC |
PW6105 | rocS1-P09:ISlacZ/hah; out of frame fusion in MPAO1; Tetr | [67], UWGC |
PW7672 | rocS2-P07::ISlacZ/hah; out of frame fusion in MPAO1; Tetr | [67], UWGC |
MPAO1ΔpvcCD | MPAO1 carrying a 600-bp BglII deletion encompassing portions of pvcC and pvcD; Tetr | This study |
Plasmids | ||
pCR®2.1-TOPO® | General cloning vector; Cbr, Kmr | Invitrogen |
pCR2.1-1.8 | pCR®2.1-TOPO® carrying the 1.8-kbp PstI P. aeruginosa stability fragment; Cbr, Kmr | This study |
pLL1 | pCR2.1-1.8 carrying intact pvcA on a 1730-bp fragment from MPAO1; Cbr, Kmr | This study |
pLL2 | pCR2.1-1.8 carrying intact pvcB on a 1200-bp fragment from MPAO1; Cbr, Kmr | This study |
pLL4 | pCR2.1-1.8 carrying intact rocS1 on a 4060-bp fragment from MPAO1; Cbr, Kmr | This study |
pLL5 | pCR2.1-1.8 carrying intact pvcAB on a 2930-bp fragment from MPAO1; Cbr, Kmr | This study |
p18.230 | pKT230 carrying pUC18; Cbr, Kmr | [39] |
pJAC7-1 | pUC19 containing ptxR on a 2.1-kbp KpnI-BglII fragment carried in pKT230; Cbr, Kmr | [39] |
pMRP9-1 | pUCP18 carrying a gene encoding enhanced green fluorescent protein (GFP); Cbr | [43] |
pAH54 | pUC18 carrying a 5.4 kb HindIII-EcoRI fragment carrying ptxR, pvcD, pvcC, and part of pvcB | [39] |
pNC80 | pAH54 with a Tet cassette replacing the 600 bp BglII fragment that carries the entire pvcDand part of pvcC; Tetr | This study |
Cb, carbenicillin;
Km, kanamycin;
, resistant;
Tet, tetracycline.