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. 2013 Apr 30;8(4):e63199. doi: 10.1371/journal.pone.0063199

Figure 3. ER66 and ER46, but not ER36, binds with 17β-estradiol at physiological concentrations.

Figure 3

(A) Specific binding of [3H]-17β-estradiol to ER66 (○) and ER46 (•) expressed in eukaryotic system with Kd values of 68.8 pM and 60.72 pM, respectively. No saturable specific binding was found for ER36 (□). (C) Specific binding of [3H]-17β-estradiol to ER66 (○) and ER46 (•) expressed in prokaryotic system with Kd values of 119.4 pM and 433.7 pM, respectively. No saturable specific binding was found for ER36 (□). (B, D) Scatchard plot analysis for ER66 and ER46 showed single-site binding. Results are representative of three experiments each done in duplicate.