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. 2013 Apr 30;8(4):e63199. doi: 10.1371/journal.pone.0063199

Table 1. Summary Table of binding affinities of 17β-estradiol to mERs.

Kd of ER66 Kd of ER46 Kd of ER36
Eukaryotic expression system (with NLP) 68.8 pM 60.7 pM no specific binding
Prokaryotic expression system (with NLP) 119.4 pM 433.7 pM no specific binding
Eukaryotic expression system (with NLP and 2-bromopalmitate) 185.0 pM 337.5 pM
Eukaryotic expression system (without NLP) 130.4 pM 399.7 pM