Table 1. Summary Table of binding affinities of 17β-estradiol to mERs.
Kd of ER66 | Kd of ER46 | Kd of ER36 | |
Eukaryotic expression system (with NLP) | 68.8 pM | 60.7 pM | no specific binding |
Prokaryotic expression system (with NLP) | 119.4 pM | 433.7 pM | no specific binding |
Eukaryotic expression system (with NLP and 2-bromopalmitate) | 185.0 pM | 337.5 pM | – |
Eukaryotic expression system (without NLP) | 130.4 pM | 399.7 pM | – |