Figure 5. Dynamics of Bcd-dependent transcription at cycle 14A.

(A) Shown are the mean ρ profiles as a function of x/L at early nuclear cycle 14A. Each color represents a time class; the adjacent t time classes differ by ∼1 min (see Fig. 2A). See Fig. S1 for additional information about the time classes shown here. For the sake of clarity, standard deviations are not shown in this figure. See Fig. S3 for standard deviations. (B) Shown are ρ_plat values for individual embryos from different time classes. The mean and standard deviation are: 0.50±0.07, 0.84±0.05, 1.03±0.07, 0.70±0.11, 0.45±0.13, 0.29±0.08, 0.18±0.07, 0.14±0.03, 0.13±0.08 for t1 to t9, respectively. The p value from Student’s t-test between t8 and t9 is 0.81, suggesting that Bcd-dependent hb transcription has reached a background level that no longer changes over time. (C) Shown are the mean profiles of raw, background-subtracted hb intensities (in arbitrary units) detecting cytoplasmic mature mRNA at different time classes of cycle 14A. Here the adjacent T time classes differ by ∼5 min (see Fig. 2A). For clarity, only mean profiles are shown. See Fig. S4 for individual profiles. (D) Shown are hb_plat values (in arbitrary units) detecting cytoplasmic mature mRNA in individual embryos from different time classes. The mean and standard deviation are: 24.03±6.37, 30.10±4.90, 33.78±5.88, 19.86±5.75 for T1 to T4, respectively.