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a. spermatozoa cryopreserved in micro-channel of which height is 10 µm (group A) one by one; b. spermatozoa cryopreserved in micro-channel of which height is 50 µm (group B); c. spermatozoa cryopreserved in micro-channel of which height is 100 µm (group C); d. spermatozoa cryopreserved disorderly in a 1.8 ml freezing tube (group D).
