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The symbol “−” and “+” represent cathode and anode respectively during electrophoresis of negatively charged DNA. a. pre-frozen spermatozoa; b. post-thawed spermatozoa of Group A; c. comet tail of post-thawed spermatozoa in group B; d. obvious long comet tail in post-thawed spermatozoa of Group C; e. post-thawed spermatozoa of Group D.
