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. 2013 Apr 30;8(4):e63205. doi: 10.1371/journal.pone.0063205

Figure 4. Ablation of CHOP protein in P30 T17M RHO retinas led to modulation of the PERK and IRE1 pathways of the UPR.

Figure 4

A: We analyzed T17M RHO and T17M RHO CHOP−/− retinas (N = 4) and found that the expression of phosphorylated eIF2α protein was increased by over 8 fold in T17M RHO CHOP−/− mice (0.5±0.1 a.u.vs. 0.04±0.1 a.u., P = 0.01). The level of spliced Xbp1 in the T17M RHO retina was decreased by 30%, presenting a value of 0.64±0.027 a.u. vs. 0.4±0.03 a.u.in T17M RHO CHOP−/− mice (P = 0.004). B: Representative images of western blots treated with antibodies against Xbp1, peIF2α and β-actin proteins.