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. 2013 Apr 30;8(4):e62319. doi: 10.1371/journal.pone.0062319

Figure 5. Polyfunctionality assay of HCV-specific T cells in the patients of group II.

Figure 5

Representative example of polyfunctionality assays of CD4+ T cells in CMI-8 is presented. PBMCs were stimulated with NS3 peptide mix, and IFN-γ, TNF-α, IL-2, and MIP-1β production were assessed. The data are presented by FACS dot plots and the pie graph shows the fraction of T cells positive for a given number of functions (A). Detailed analysis of polyfunctionality is presented with every possible combination of functions (B).