Table 4. Real-time PCR data for M. leprae screening methods (RLEP & 18-kD) and for MTB complex DNA (IS1081).

Burial	Extract	RLEP PCR	Cq values1 (RLEP)	18-kD PCR	Cq values1 (18-kD)	IS1081 PCR
Sk1	Palate	−	No Cq	−	No Cq	−
	Nasal conchae	−	No Cq	−	No Cq	−
Sk2	Foot phalanx	++	28.09	nd	nd	−
	Cranial fragment	++	26.5	+	32.78	−
Sk7	Foot phalanx	+	37.0	−	No Cq	−
	Nasal conchae	+	32.33	±	38.59	−
	Ossified tibial ligament	±	38.74	−	No Cq	−
Sk8	R Fibula 1 new bone	++	25.08	nd	nd	−
	R Fibula 2 next to 1	+	32.20	±	38.43	−
	L maxilla	+	29.55	+	35.68	−
	Ethmoid bone	+	36.01	−	No Cq	−
Sk9	Foot phalanx	−	No Cq	nd	nd	−
	L tibia, proximal end	±	37.47	nd	nd	−
Sk12	Nasal conchae	−	No Cq	−	No Cq	−
Sk14	Cranial fragment	++a	23.71	++	28.75	−
	Nasal conchae	++	27.91	+	34.22	−
	Metatarsal	++	27.11	+	33.98	−
Sk152	R fibula	±	37.77	−	No Cq	−
	Vertebra (1) T11	−	No Cq	−	No Cq	−
	Vertebra (2) T11	−	No Cq	−	No Cq	−
	Rib	−	No Cq	−	No Cq	−
Sk18	R fibula	±a	38.46	−	No Cq	−
	Cranial fragment	±	38.86	−	No Cq	−
Sk19	R foot	±a	32.38	±	37.3	−
	R metacarpal (1)	+	34.5	±	38.14	−
	R metacarpal (2)	+	34.49	±	38.52	−
	Dental calculus	+	36.29	−	No Cq	−
Sk23	R 4th metatarsal	±a	37.99	±	38.6	−
	R. fibula	±	38.4	−	No Cq	−

¹ = Mean of duplicates.

² = Osteological evidence of tuberculosis or brucellosis.

^a = RLEP PCR confirmed at the second centre.

Scoring scheme for individual cases: ± = weak positive;+ = positive;++ = strong positive.